Deleted
Sample Discontinued
Experience: None
No Sequence
Get This Part
DNA

Part:BBa_K2100062

Designed by: Trinh Nguyen   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH: TP901

This is a final expression vector that we used to transfect into mammalian cells for our experiments. TP901 is a serine recombinase, which we used as biological latches to control expression of a final out put gene for our genetic circuit. pEGSH is an inducible promoter similar to pTRE. This inducible promoter system require the presence of VgEcr and RXR protein and small molecule Ponasterone A (PonA). By putting TP901 recombinase under pEGSH, we can control the expression of TP901 with small molecule PonA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None